Newcastle Falcons wing Adam Radwan will make his England debut against Canada on Saturday, with club-mate Jamie Blamire starting and Callum Chick being named on the bench.
The Twickenham clash is a 3pm kick-off and will be shown live in the UK on Channel 4.
Radwan earns his first cap after a stellar season in which he scored seven tries in 11 games, taking his overall tally in Falcons colours to 36 tries in 46 games.
Hooker Blamire scored on his England debut last weekend just three minutes after coming on from the bench in the 43-29 victory over the USA, and is given a chance to impress from the start on Saturday.
Chick started the USA game at No 8 to win his first cap, with prop-forward Trevor Davison also coming on for his test debut during the closing quarter.
England head coach Eddie Jones said: “Our focus this camp is being as strong a 36-player squad as we can be, and there has been a lot of hard work and intense competition.
“There is a responsibility on the 23 players selected to perform to their personal best.
“We want to go out there and put smiles on the faces of all the supporters watching at Twickenham and at home on Channel 4.”
15 Freddie Steward (Leicester Tigers, 1 cap)
14 Joe Cokanasiga (Bath Rugby, 10 caps)
13 Henry Slade (Exeter Chiefs, 39 caps)
12 Dan Kelly (Leicester Tigers, uncapped)
11 Adam Radwan (Newcastle Falcons, uncapped)
10 Marcus Smith (Harlequins, 1 cap)
9 Harry Randall (Bristol Bears, 1 cap)
1 Ellis Genge (Leicester Tigers, 29 caps)
2 Jamie Blamire (Newcastle Falcons, 1 cap)
3 Joe Heyes (Leicester Tigers, 1 cap)
4 Harry Wells (Leicester Tigers, uncapped)
5 Charlie Ewels (Bath Rugby, 22 caps)
6 Lewis Ludlow (Gloucester Rugby, captain 1 cap)
7 Sam Underhill (Bath Rugby, 23 caps)
8 Alex Dombrandt (Harlequins, uncapped)
16 Curtis Langdon (Sale Sharks, 1 cap)
17 Beno Obano (Bath Rugby, 2 caps)
18 Paul Hill (Northampton Saints, 5 caps)
19 Callum Chick (Newcastle Falcons, 1 cap)
20 Lewis Ludlam (Northampton Saints, 9 caps)
21 Dan Robson (Wasps, 13 caps)
22 George Furbank (Northampton Saints, 3 caps)
23 Joe Marchant (Harlequins, 5 caps)
PHOTO CAPTION: (left to right) Adam Radwan, Jamie Blamire and Callum Chick